November 12, 2014
Notes: Yanar, Fatih
Sarici, Inanc S
Gok, Ali F K
Int J Surg Case Rep. 2013;4(8):708-10. doi: 10.1016/j.ijscr.2013.02.025. Epub 2013 Apr 17.
Author Address: Department of General Surgery, Trauma & Emergency Surgery Service, Istanbul University, Istanbul Medical Faculty, Turkey. Electronic address: email@example.com.
Reference Type: Journal Article
Record Number: 5319Author: Yang, Y. S., Chen, K., Ye, S., Shi, X. L., Du, Z. W., Cui, S. L. and Wang, H.
Title: [Differentially expressed proteins of severe acute pancreatitis intervened by Qingyi granule]
Journal: Zhongguo Zhong Xi Yi Jie He Za Zhi
Short Title: [Differentially expressed proteins of severe acute pancreatitis intervened by Qingyi granule]
Alternate Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban
ISSN: 1003-5370 (Print)
Accession Number: 23596789
Disease Models, Animal
Drugs, Chinese Herbal/*therapeutic use
Pancreatitis/chemically induced/*metabolism/*prevention & control
Abstract: OBJECTIVE: To observe the effects of Qingyi Granule (QYG) on the changes of total protein expressions in the pancreatic tissue of rats with severe acute pancreatitis (SAP) induced by sodium taurocholate (STC). METHODS: SAP was induced by retrograded injecting 5% STC from the gut-pancreatic duct in 36 Sprague-Dawley (SD)rats. Then they were randomly divided into the SAP group and the QYG treatment group (abbreviated as the QYG group), 18 in each group. After successful modeling, rats in the QYG group were administered with QYG water solution (W: W = 1:1) once with an interval of 12 h (1 mL/100 g), while rats in the SAP group were administered with normal saline. The medication was performed four times. The total proteins were extracted from the pancreatic tissue of all rats to perform two-dimensional electrophoresis, fluorescent staining, and atlas analysis. The protein dots with differential expressions more than four times between each other in 48 h gel pictures were chosen and used for MALDI-TOF/TOF mass chromatographic analysis and biological information analysis. RESULTS: The 5% STC induced SAP model rats had typical pathological changes in the pancreatic tissue. The proteomics changes of the pancreatic tissue were analyzed by gel image manipulation software. Twenty two disparate points were detected between two groups at 48 h, 5 points of the protein were up-regulated and 17 points were down-regulated of the total after QYG intervention. Nine protein spots expressed differently more than 4 times and stably at 48 h, 7 kinds of proteins have been identified by mass chromatographic analysis and Data Base Retrieval, and they were Serpinb1a 39 kDa protein, Serpinb1a 43 kDa protein, Prdx4 Prx IV, Clps, gamma-actin (Actg1), Eprs and Hadhsc. Those proteins were involved in signal transmit during the process of SAP pancreas–pathological injury analyzed from their functions. CONCLUSIONS: Proteomics can well reflect the effects of QYG on differential expression proteins in the pancreatic tissue of rats with SAP. Studying differential expression proteins may provide a new theoretical basis and molecule target for QYG treating SAP.