November 12, 2014
Notes: Spanier, B W Marcel
Bruno, Marco J
Dijkgraaf, Marcel G W
Research Support, Non-U.S. Gov’t
World J Gastroenterol. 2013 May 28;19(20):3018-26. doi: 10.3748/wjg.v19.i20.3018.
Author Address: Department of Gastroenterology and Hepatology, Rijnstate Hospital, 6800 TA Arnhem, The Netherlands. firstname.lastname@example.org
Reference Type: Journal Article
Record Number: 5261Author: Sri Manjari, K., Nallari, P., Balakrishna, N., Vidyasagar, A., Prabhakar, B., Jyothy, A. and Venkateshwari, A.
Title: Influence of matrix metalloproteinase-1 gene -1607 (1G/2G) (rs1799750) promoter polymorphism on circulating levels of MMP-1 in chronic pancreatitis
Journal: Biochem Genet
Short Title: Influence of matrix metalloproteinase-1 gene -1607 (1G/2G) (rs1799750) promoter polymorphism on circulating levels of MMP-1 in chronic pancreatitis
Alternate Journal: Biochemical genetics
ISSN: 1573-4927 (Electronic)
Accession Number: 23644943
*Gene Expression Regulation, Enzymologic
Matrix Metalloproteinase 1/*genetics
*Polymorphism, Single Nucleotide
*Promoter Regions, Genetic
Sequence Analysis, DNA
Abstract: This study investigated the role of -1607 (1G/2G) (rs1799750) polymorphism of the MMP-1 gene in chronic pancreatitis. We genotyped 100 patients with chronic pancreatitis and 100 control subjects using tetra-primer ARMS-PCR followed by agarose gel electrophoresis. Serum levels of MMP-1 were determined by Elisa. Statistical analysis was applied to test the significance of the results. The genotypic and allelic distribution varied significantly between the disease group and the control subjects [OD = 1.981 (1.236-3.181), p = 0.004]. MMP-1 levels were higher in subjects homozygous for the 2G allele than in subjects with the 1G allele. The present study revealed a significant association of the MMP-1 -1607 1G/2G (rs1799750) gene promoter polymorphism with chronic pancreatitis, and it can be considered a biological marker in the etiology of chronic pancreatitis.