November 12, 2014
Notes: Prozorow-Krol, Beata
Arch Pharm Res. 2013 Sep;36(9):1126-32. doi: 10.1007/s12272-013-0126-2.
Author Address: Department of Gastroenterology with Endoscopic Unit, Medical University of Lublin, Jaczewski Street 8, 20-954, Lublin, Poland.
Reference Type: Journal Article
Record Number: 5093Author: Qin, Y., Pinhu, L., You, Y., Sooranna, S., Huang, Z., Zhou, X., Yin, Y. and Song, S.
Title: The role of Fas expression on the occurrence of immunosuppression in severe acute pancreatitis
Journal: Dig Dis Sci
Short Title: The role of Fas expression on the occurrence of immunosuppression in severe acute pancreatitis
Alternate Journal: Digestive diseases and sciences
ISSN: 1573-2568 (Electronic)
Accession Number: 23861115
Gene Expression Regulation/*immunology
Abstract: BACKGROUND: Severe acute pancreatitis (SAP) is a dangerous illness with high mortality where most patients do not die of excessive inflammation, but die of immunosuppression and multiple infections at a later stage. The mechanism of immunosuppression in SAP is unknown. AIM: The purpose of this study was to analyze the role of Fas expression on the occurrence of immunosuppression in patients with SAP. METHODS: Forty-eight patients with pancreatitis were divided into two groups: 20 cases with SAP (7 cases with sepsis, 13 cases without sepsis) and 28 cases with mild acute pancreatitis (MAP). Twenty-eight healthy volunteers were selected as controls. Fas mRNA expression in peripheral blood was detected by qPCR and Fas protein of lymphocyte membranes; T lymphocyte subsets and expression of monocyte Human leukocyte antigen DR (HLA-DR) in peripheral blood were detected by flow cytometry. RESULTS: Compared with MAP and control groups, expression level of Fas mRNA and lymphocyte Fas protein in peripheral blood were significantly increased in the SAP group (all P < 0.01). There was a further significant increase in the SAP group with sepsis compared to those without sepsis (all P < 0.01). The CD4(+) T cell ratio, CD4(+)/CD8(+) ratio and monocyte HLA-DR expression in the SAP group were decreased significantly compared with MAP and control groups (all P < 0.01). Significant negative relationships were observed between Fas mRNA expression and CD4(+) T-cell ratio, CD4(+)/CD8(+) ratio, and monocyte HLA-DR expression in SAP patients with sepsis (all P < 0.05). CONCLUSIONS: The results suggest that expression level of Fas is related to severity and immune status of pancreatitis. Overexpression of Fas may lead to the occurrence of immunosuppression and sepsis.