November 12, 2014
Notes: Mazaki, Takero
J Gastroenterol. 2014 Feb;49(2):343-55. doi: 10.1007/s00535-013-0806-1. Epub 2013 Apr 24.
Author Address: Department of Surgery, Nihon University School of Medicine, 30-1 Oyaguchikami-machi, Itabashi-ku, Tokyo, 173-8610, Japan, email@example.com.
Reference Type: Journal Article
Record Number: 5191Author: Meng, H., Gong, J., Fang, L., Song, Z., Wu, F., Zhou, B. and Qian, M.
Title: Effect of interferon-gamma on NF-kappaB and cytokine IL-18 and IL-27 in acute pancreatitis
Journal: Bosn J Basic Med Sci
Short Title: Effect of interferon-gamma on NF-kappaB and cytokine IL-18 and IL-27 in acute pancreatitis
Alternate Journal: Bosnian journal of basic medical sciences / Udruzenje basicnih mediciniskih znanosti = Association of Basic Medical Sciences
ISSN: 1840-4812 (Electronic)
Accession Number: 23725508
Abstract: The objective of our work was to evaluate the effect of interferon-gamma (IFN-gamma) on cytokine expression in rat acute pancreatitis (AP). AP was introduced to rats which were divided into Control, AP and IFN-gamma group. Rats in the AP and IFN-gamma group were sacrificed as 6, 12 and 24 h after IFN-gamma treatment. The serum amylase (AMA), endotoxin and cytokines were detected. The pathological examination and immunofluorescence staining of pancreas for TNF-alpha, NF-kappaB and IL-18 were performed. The serum AMA increased significantly at 6 h and reduced at 48 h after AP. The increase in IFN-gamma was higher than that in AMA. IL-18 increased in the AP and IFN group, and IFN increased markedly at 48 h after AP. IL-27 reduced at 24 h after AP compared with AP group. In the AP group, the immunostaining of cytokines increased. In the IFN group, the edema in the pancreas was more severe, and NF-kappaB and IL-18 expression was higher than that in the other two groups. IFN-gamma can increase serum IL-18 and reduce IL-27 in AP. IFN-gamma can increase serum IL-18 and reduce serum IL-27 in AP. The increase in NF-kappaB and IL-18 may exert influence on pro-inflammatory cytokines to deteriorate inflammation in the pancreas. Thus, to control the IFN-gamma might has promise to attenuate pancreatitis.