November 12, 2014
Notes: Lohr, J-Matthias
Haas, Stephen L
Dig Dis. 2013;31(1):43-50. doi: 10.1159/000345720. Epub 2013 Jun 17.
Author Address: Gastroenterology and Hepatology, Gastrocentrum, Karolinska University Hospital, Stockholm, Sweden. firstname.lastname@example.org
Reference Type: Journal Article
Record Number: 5313Author: Luan, Z. G., Zhang, J., Yin, X. H., Ma, X. C. and Guo, R. X.
Title: Ethyl pyruvate significantly inhibits tumour necrosis factor-alpha, interleukin-1beta and high mobility group box 1 releasing and attenuates sodium taurocholate-induced severe acute pancreatitis associated with acute lung injury
Journal: Clin Exp Immunol
Short Title: Ethyl pyruvate significantly inhibits tumour necrosis factor-alpha, interleukin-1beta and high mobility group box 1 releasing and attenuates sodium taurocholate-induced severe acute pancreatitis associated with acute lung injury
Alternate Journal: Clinical and experimental immunology
ISSN: 1365-2249 (Electronic)
Accession Number: 23600830
Keywords: Active Transport, Cell Nucleus/drug effects
Acute Lung Injury/*drug therapy/etiology/*immunology/pathology
Anti-Inflammatory Agents, Non-Steroidal/therapeutic use
HMGB1 Protein/*antagonists & inhibitors
Interleukin-1beta/*antagonists & inhibitors
Tumor Necrosis Factor-alpha/*antagonists & inhibitors
Abstract: In this study, we examined the effect of ethyl pyruvate (EP) on pulmonary inflammation in rats with severe pancreatitis-associated acute lung injury (ALI). Severe acute pancreatitis (SAP) was induced in rats by the retrograde injection of 5% sodium taurocholate into the pancreatic duct. Rats were randomly divided into the following experimental groups: control group, SAP group and EP-treated group. The tissue specimens were harvested for morphological studies, Streptavidin-peroxidase immunohistochemistry examination. Pancreatic or lung tissue oedema was evaluated by tissue water content. Serum amylase and lung tissue malondialdehyde (MDA) and myeloperoxidase (MPO) were measured. Meanwhile, the nuclear factor-kappaB (NF-kappaB) activation, tumour necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) levels and HMGB1 protein expression levels in the lung were studied. In the present study, we demonstrated that treatment with EP after SAP was associated with a reduction in the severity of SAP and lung injury. Treatment with EP significantly decreased the expression of TNF-alpha, IL-1beta, HMGB1 and ameliorated MDA concentration, MPO activity in the lung in SAP rats. Compared to SAP group, administration of EP prevented pancreatitis-induced increases in nuclear translocation of NF-kappaB in the lung. Similarly, treatment with EP significantly decreased the accumulation of neutrophils and markedly reduced the enhanced lung permeability. In conclusion, these results demonstrate that EP might play a therapeutic role in pulmonary inflammation in this SAP model.