November 12, 2014
Notes: Han, Song
Englander, Ella W
Gomez, Guillermo A
Aronson, Judith F
Garofalo, R P
Greeley, George H Jr
R01 DK-035608/DK/NIDDK NIH HHS/
Research Support, N.I.H., Extramural
Am J Physiol Gastrointest Liver Physiol. 2013 Jul 15;305(2):G139-50. doi: 10.1152/ajpgi.00370.2012. Epub 2013 May 16.
Author Address: Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USA.
Reference Type: Journal Article
Record Number: 5158Author: Han, T., Li, X. L., Cai, D. L., Zhong, Y. and Geng, S. S.
Title: Effects of glutamine-supplemented enteral or parenteral nutrition on apoptosis of intestinal mucosal cells in rats with severe acute pancreatitis
Journal: Eur Rev Med Pharmacol Sci
Short Title: Effects of glutamine-supplemented enteral or parenteral nutrition on apoptosis of intestinal mucosal cells in rats with severe acute pancreatitis
Alternate Journal: European review for medical and pharmacological sciences
ISSN: 1128-3602 (Print)
Accession Number: 23771542
Keywords: Acute Disease
Glutamine/*administration & dosage
bcl-2-Associated X Protein/analysis
Abstract: BACKGROUND: Hhigh mortality of the severe acute pancreatitis (SAP) is caused by the damage of intestinal mucosal barrier. Glutamine (Gln) has been used to protect the intestinal mucosal barrier in the treatments of many severe diseases. AIM: To explore the impact of glutamine on the apoptosis of intestinal mucosa and Bax expression in rats with SAP. MATERIALS AND METHODS: Eighty male SD rats were randomly divided into five groups: sham-operated group, SAP + parenteral nutrition (PN) group, SAP + enteral nutrition (EN) group, SAP + EN + Gln group, and SAP + PN + Gln group. Rats were sacrificed 4 days and 7 days after nutritional support. The intestinal epithelial apoptosis and the expression of Bax were examined by TUNEL assay and immunohistochemical staining, respectively. RESULTS: The apoptotic index (AI) of SAP groups was higher than that of sham-operated group. After 7 days treatment, the AI of SAP + EN + Gln group was significantly lower than that of the SAP + EN or SAP + PN group. In addition, the AI of the SAP groups after 7 days treatment was significantly lower than that of the same groups at 4 days after treatment. Furthermore, the Bax expression of SAP + EN + Gln group was significantly lower than that in the SAP + EN or SAP + PN group. However, no significant differences were observed between SAP + EN + Gln group and SAP + PN + Gln group in AI and Bax expression. CONCLUSIONS: Combination of Gln and parenteral nutrition or enteral nutrition inhibits the apoptosis of intestinal epithelial cells and maintains the integrity of the intestinal mucosal barrier.