November 12, 2014
Notes: Zaheer, Atif
Halappa, Vivek Gowdra
Akshintala, Venkata S
Singh, Vikesh K
Kamel, Ihab R
JOP. 2013 Sep 10;14(5):506-9. doi: 10.6092/1590-8577/1400.
Author Address: Russell H Morgan Department of Radiology and Radiological Science, Johns Hopkins Medical Institutions. Baltimore, MD, USA. firstname.lastname@example.org.
Reference Type: Journal Article
Record Number: 4868Author: Zechner, D., Sempert, K., Genz, B., Timm, F., Burtin, F., Kroemer, T., Butschkau, A., Kuhla, A. and Vollmar, B.
Title: Impact of hyperglycemia and acute pancreatitis on the receptor for advanced glycation endproducts
Journal: Int J Clin Exp Pathol
Short Title: Impact of hyperglycemia and acute pancreatitis on the receptor for advanced glycation endproducts
Alternate Journal: International journal of clinical and experimental pathology
ISSN: 1936-2625 (Electronic)
Accession Number: 24133579
Diabetes Mellitus, Experimental/*metabolism/pathology
MAP Kinase Signaling System/physiology
Mice, Inbred C57BL
Abstract: Since hyperglycemia aggravates acute pancreatitis and also activates the receptor for advanced glycation endproducts (RAGE) in other organs, we explored if RAGE is expressed in the pancreas and if its expression is regulated during acute pancreatitis and hyperglycemia. Acute pancreatitis was induced by cerulein in untreated and streptozotocin treated diabetic mice. Expression of RAGE was analyzed by Western blot and immunohistochemistry. To evaluate signal transduction the phosphorylation of ERK1/ERK2 was assessed by Western blot and the progression of acute pancreatitis was monitored by evaluation of lipase activity and the pancreas wet to dry weight ratio. RAGE is mainly expressed by acinar as well as interstitial cells in the pancreas. During acute pancreatitis infiltrating inflammatory cells also express RAGE. Using two distinct anti-RAGE antibodies six RAGE proteins with diverse molecular weight are detected in the pancreas, whereas just three distinct RAGE proteins are detected in the lung. Hyperglycemia, which aggravates acute pancreatitis, significantly reduces the production of two RAGE proteins in the inflamed pancreas.