November 12, 2014
Notes: Popa, D
J Med Life. 2013 Sep 15;6(3):307-9. Epub 2013 Sep 25.
Author Address: “Carol Davila” University of Medicine and Pharmacy, Department of Surgery, Bucharest University Emergency Hospital, Bucharest, Romania.
Reference Type: Journal Article
Record Number: 4942Author: Poxleitner, P. J., Seifert, G., Richter, S. C., Hopt, U. T. and Wittel, U. A.
Title: Infected pancreatic necrosis increases the severity of experimental necrotizing pancreatitis in mice
Short Title: Infected pancreatic necrosis increases the severity of experimental necrotizing pancreatitis in mice
Alternate Journal: Pancreas
ISSN: 1536-4828 (Electronic)
Accession Number: 24048455
Disease Models, Animal
Escherichia coli Infections/microbiology/*pathology
Mice, Inbred BALB C
Pancreatitis, Acute Necrotizing/*microbiology/*pathology
Systemic Inflammatory Response Syndrome/microbiology/pathology
Abstract: OBJECTIVE: Infection of pancreatic necrosis in necrotizing pancreatitis increases the lethality of patients with acute pancreatitis. To examine mechanisms underlying this clinical observation, we developed and tested a model, in which primary infection of necrosis is achieved in taurocholate-induced pancreatitis in mice. METHODS: Sterile necrosis of acute necrotizing pancreatitis was induced by retrograde injection of 4% taurocholate into the common bile duct of Balb/c mice. Primary infection of pancreatic necrosis was induced by coinjecting 10 colony-forming units of Escherichia coli. Animals were killed after 6, 12, 24, 48, and 120 hours, and pancreatic damage and pancreatitis-associated systemic inflammatory response were assessed. RESULTS: Mice with pancreatic acinar cell necrosis had an increased bacterial concentration in all tissues and showed sustained bacteremia. Acute pancreatitis was induced only by coinjection of taurocholate and not by bacterial infection alone. Infection of pancreatic necrosis increased pancreatic damage and the pulmonary vascular leak. Serum glucose concentrations serving as a parameter of hepatic function were reduced in mice with infected pancreatic necrosis. CONCLUSIONS: Primary infection of pancreatic necrosis with E. coli increases both pancreatic damage and pulmonary and hepatic complications in acute necrotizing pancreatitis in mice.