November 12, 2014
Journal: Dig Endosc
Short Title: Endoscopic ultrasonography-guided trans-Roux limb drainage of pancreatic duct to prevent pancreatitis as a result of percutaneous metallic stent placement for malignant biliary stricture
Alternate Journal: Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society
ISSN: 1443-1661 (Electronic)
Accession Number: 24118187
Keywords: Bile Ducts/*pathology
Pancreatitis/*prevention & control
Notes: Ono, Michihiro
Dig Endosc. 2014 Jan;26(1):120-1. doi: 10.1111/den.12177. Epub 2013 Sep 30.
Author Address: Depertment of Medical Oncology and Hematology, Sapporo Medical University, Sapporo, Japan.
Reference Type: Journal Article
Record Number: 5075Author: Oppliger, S., Hartnack, S., Riond, B., Reusch, C. E. and Kook, P. H.
Title: Agreement of the serum Spec fPL and 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester lipase assay for the determination of serum lipase in cats with suspicion of pancreatitis
Journal: J Vet Intern Med
Short Title: Agreement of the serum Spec fPL and 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester lipase assay for the determination of serum lipase in cats with suspicion of pancreatitis
Alternate Journal: Journal of veterinary internal medicine / American College of Veterinary Internal Medicine
ISSN: 1939-1676 (Electronic)
Accession Number: 23888903
Abstract: BACKGROUND: Serum lipase activities measured by catalytic assays are claimed to be of limited utility for diagnosing pancreatitis in cats. The Spec fPL assay currently is believed the most sensitive test; however, studies comparing different lipase assays are lacking. 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester (DGGR) assay for the determination of lipase activity has been evaluated in dogs, but no information is available in cats. OBJECTIVES: To investigate the agreement of DGGR-lipase activity and Spec fPL concentration in cats with clinical signs consistent with pancreatitis. ANIMALS: Two hundred fifty-one client-owned cats. METHODS: DGGR-lipase activity and Spec fPL concentration measured from the same blood sample in cats undergoing investigation for pancreatitis. The agreement between DGGR-lipase and Spec fPL at different cutoffs was assessed using Cohen’s kappa coefficient (kappa). Sensitivity and specificity were calculated for 31 cases where pancreatic histopathology was available. RESULTS: DGGR-lipase (cutoff, 26 U/L) and Spec fPL (cutoff, >5.3 mug/L) had a kappa of 0.68 (standard error [SE] 0.046). DGGR-lipase (cutoff, 26 U/L) and Spec fPL (cutoff, >3.5 mug/L) had a kappa of 0.60 (SE, 0.05). The maximum kappa at a Spec fPL cutoff >5.3 mug/L was found when the DGGR-lipase cutoff was set >34 U/L and calculated as 0.755 (SE, 0.042). Sensitivity and specificity were 48% and 63% for DGGR-lipase (cut-off, 26 U/L) and 57% and 63% for Spec fPL (>5.3 mug/L), respectively. CONCLUSIONS AND CLINICAL IMPORTANCE: Both lipase assays agreed substantially. DGGR assay seems a useful and cost-efficient method compared to the Spec fPL test.