November 12, 2014
Notes: Carrasco, Cristina
Holguin-Arevalo, Maria S
Rodriguez, Ana B
Pariente, Jose A
Research Support, Non-U.S. Gov’t
Mol Cell Biochem. 2014 Feb;387(1-2):217-25. Epub 2013 Nov 15.
Reference Type: Journal Article
Record Number: 4628Author: Carrasco, C., Marchena, A. M., Holguin-Arevalo, M. S., Martin-Partido, G., Rodriguez, A. B., Paredes, S. D. and Pariente, J. A.
Title: Anti-inflammatory effects of melatonin in a rat model of caerulein-induced acute pancreatitis
Journal: Cell Biochem Funct
Short Title: Anti-inflammatory effects of melatonin in a rat model of caerulein-induced acute pancreatitis
Alternate Journal: Cell biochemistry and function
ISSN: 1099-0844 (Electronic)
Accession Number: 24779037
Keywords: Acute Disease
Anti-Inflammatory Agents/*therapeutic use
Pancreatitis/chemically induced/*drug therapy/pathology
Abstract: The purpose of our study was to evaluate the protective effect of melatonin in a rat model of caerulein-induced acute pancreatitis. For the induction of experimental acute pancreatitis, four subcutaneous injections of caerulein (20 mgkg-1 body weight) were given to Wistar rats at 2-h intervals. Melatonin was injected intraperitoneally (25 mg kg-1 body weight) 30 min before each caerulein injection. After 12 h, rats were sacrificed by decapitation. Blood and pancreas samples were collected and processed for serological and histopathological studies,respectively. Lipase, a-amylase, corticosterone, total antioxidant power and cytokines interleukin (IL)-1b, IL-4 and tumour necrosis factor(TNF)-a were determined using commercial kits. ANOVA and Tukey tests (P<0.05) were performed for the statistical analysis of the results.Results showed that the administration of melatonin reduced histological damage induced by caerulein treatment as well as the hyperamylasemia and hyperlipidemia. Corticosterone and antioxidant total power were also reverted to basal activities. Furthermore, melatonin pre-treatment reduced pro-inflammatory cytokines IL-1b and TNF-a and increased the serum levels of anti-inflammatory cytokine IL-4. In conclusion,the findings suggest that the protective effect of melatonin in caerulein-induced acute pancreatitis is mediated by the anti-inflammatory ability of this indolamine. Thus, melatonin may have a protective effect against acute pancreatitis.