November 12, 2014
Notes: Leonardi, Salvatore
Pratico, Andrea Domenico
Di Dio, Giovanna
La Rosa, Mario
J Med Case Rep. 2013 Jul 24;7:188. doi: 10.1186/1752-1947-7-188.
Author Address: Department of Medical and Pediatric Science, Unit of Broncho-Pneumology and Cystic Fibrosis, University of Catania, Via Santa Sofia 78, Catania 95123, Italy. firstname.lastname@example.org.
Reference Type: Journal Article
Record Number: 5017Author: Li, B., Luo, C., Chowdhury, S., Gao, Z. H. and Liu, J. L.
Title: Parp1 deficient mice are protected from streptozotocin-induced diabetes but not caerulein-induced pancreatitis, independent of the induction of Reg family genes
Journal: Regul Pept
Date: Sep 10
Short Title: Parp1 deficient mice are protected from streptozotocin-induced diabetes but not caerulein-induced pancreatitis, independent of the induction of Reg family genes
Alternate Journal: Regulatory peptides
ISSN: 1873-1686 (Electronic)
Accession Number: 23954400
Diabetes Mellitus, Experimental/chemically induced/*enzymology
Glucose Transporter Type 2/genetics/metabolism
Mice, Inbred ICR
Abstract: Poly(ADP-ribose) polymerase (Parp) 1 is a key regulator of cell death, its inhibition prevented streptozotocin-induced diabetes and attenuated caerulein-induced acute pancreatitis. Reg family proteins are significantly induced by Parp1 inhibitor, experimental diabetes and/or acute pancreatitis. We propose that Reg proteins are involved in the protection of pancreatic cells by Parp1 inhibition. To test this possibility, Parp1-/- and wild-type mice were injected with streptozotocin to induce diabetes. Separately, acute pancreatitis was induced with repeated injections of caerulein. Upon streptozotocin administration, Parp1-/- mice displayed much decreased hyperglycemia and preserved serum insulin level. The treatment induced similar levels of Reg1, -2, -3alpha and -3beta genes in the pancreas of both wild-type and Parp1-/- mice, suggesting that the upregulation of Reg family genes during streptozotocin-induced diabetes was independent of Parp1 ablation. In caerulein-induced pancreatitis, unlike being reported, Parp1 knockout caused no relief on the severity of pancreatitis; the upregulation of pancreatic Reg1, -2, -3alpha and -3beta genes upon caerulein was unaffected by Parp1 deletion. Our results reconfirmed the protective effect of Parp1 gene deletion on islet beta-cells but questioned its effect on the acinar cells. In either case, the significant induction of Reg family genes seemed independent of Parp1-mediated cell death.