November 12, 2014
Notes: Gu, H
Whitcomb, D C
Buchler, M W
Research Support, Non-U.S. Gov’t
Cell Death Dis. 2013 Oct 3;4:e816. doi: 10.1038/cddis.2013.354.
Author Address: Department of General, Visceral and Transplantation Surgery, Heidelberg, Germany.
Reference Type: Journal Article
Record Number: 5072Author: Gulcubuk, A., Haktanir, D., Cakiris, A., Ustek, D., Guzel, O., Erturk, M., Karabagli, M., Akyazi, I., Cicekci, H., Altunatmaz, K., Uzun, H. and Ates, K.
Title: Effects of curcumin on proinflammatory cytokines and tissue injury in the early and late phases of experimental acute pancreatitis
Short Title: Effects of curcumin on proinflammatory cytokines and tissue injury in the early and late phases of experimental acute pancreatitis
Alternate Journal: Pancreatology : official journal of the International Association of Pancreatology
ISSN: 1424-3911 (Electronic)
Accession Number: 23890132
Enzyme Activation/drug effects
NF-kappa B/antagonists & inhibitors
Pancreatitis, Acute Necrotizing/chemically induced/*pathology/prevention &
Transcription Factor AP-1/antagonists & inhibitors
Abstract: BACKGROUND & AIMS: Acute pancreatitis (AP) varies from mild to severe necrotizing changes with high mortality. The objective of the current study was to investigate the effects of curcumin on tissue injury and proinflammatory cytokines in the early and late phases of AP. METHODS: AP was induced by sodium taurocholate in rats (n = 140). First group was left untreated. Group II received 100 mg/kg curcumin daily starting 20 days before AP induction. The rats were allocated into 7 sub-groups (n:5) and were sacrificed at 2, 6, 12, 24, 72, 144 and 288 h following the induction of AP. Blood and pancreatic tissue samples were collected for biochemical and histopathologic evaluations and the assessment of protein and mRNA levels, as well. RESULTS: Curcumin decreased total histopathologic scores in comparison with those of the taurocholate group (P < 0.05). Curcumin increased Caspase-3 activity and decreased trypsin activity, while inhibited nuclear factor-kappa (NF-kappaB) at all time points (P < 0.05) and moreover reduced activator protein-1 (AP-1). Curcumin decreased chemokine (except for 288 h), TNF-alpha (except for 2 and 24 h), IL-6 (except for 2, 6 and 288 h) and iNOS (except for 144 and 288 h) mRNA levels (P < 0.05). Curcumin serum nitric oxide (NO) (except for 144 and 288 h) levels were reduced, as well. CONCLUSIONS: In conclusion, curcumin reduced tissue injury, trypsin activation and inhibited NF-kappaB and AP-1. However TNF-alpha, IL-6 and iNOS and NO were not inhibited at all time points. Therefore no direct correlation was detected in the subgroups between tissue injury, proinflammatory cytokines and oxidative enzymes.