November 12, 2014
Notes: Bouwense, S A
Ahmed Ali, U
ten Broek, R P
van Eijck, C H
Wilder-Smith, O H
van Goor, H
Br J Surg. 2013 Dec;100(13):1797-804. doi: 10.1002/bjs.9322.
Author Address: Department of Surgery, Rotterdam, The Netherlands.
Reference Type: Journal Article
Record Number: 4621Author: Bozkurt, S., Guner, A., Kadioglu, H., Kece, C., Reis, E. and Coskun, H.
Title: The effects of different mechanisms on the development of post-ERCP pancreatitis in an ERCP model in rats
Journal: Turk J Gastroenterol
Short Title: The effects of different mechanisms on the development of post-ERCP pancreatitis in an ERCP model in rats
Alternate Journal: The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology
ISSN: 1300-4948 (Print)
Accession Number: 24623284
Abstract: BACKGROUND/AIMS: To investigate the effects of different mechanisms on the development of pancreatitis after endoscopic retrograde cholangiopancreatography. MATERIAL AND METHODS: 40 male rats were randomly divided into four groups. After laparotomy, in Group 1, only duodenum was reached by a 24G cannula without performing any other procedure. In Groups 2, 3, and 4, biliopancreatic duct was cannulated transduodenally. Group 2 received no additional intervention after the cannulation. Group 3 received saline, whereas Group 4 received contrast agent into the duct. After a period of 24 hours, all rats were sacrificed. Laboratorytests for blood samples were performed and pancreatic tissue was also evaluated histopathologically. RESULTS: Leukocyte, blood sugar, serum glutamic oxaloacetic transaminase, lactate dehydrogenase, amylase, C-reactive protein, and base excess parameters were evaluated. The values in Groups 2, 3, and 4 were found to be significantly higher than those in the control group, except for leukocyte count and base excess (p=0.551, p=0.031, p=0.0001, p=0.0001, p=0.0001, p=0.0001, p=0.683, respectively). Histopathological results demonstrated significant differences between the groups. Highest pathological damage scores were observed in Groups 3 and 4. CONCLUSION: Among different theories for the pathogenesis of post-endoscopic retrograde cholangiopancreatography pancreatitis, elevated intraductal hydrostatic pressure was observed to be the main underlying cause.