November 12, 2014
Notes: Zhang, K-J
Research Support, Non-U.S. Gov’t
Eur Rev Med Pharmacol Sci. 2013 Dec;17(24):3279-84.
Author Address: General Surgery, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shangdong, China. firstname.lastname@example.org.
Reference Type: Journal Article
Record Number: 4835Author: Zhang, S. K., Cui, N. Q., Zhuo, Y. Z., Li, D. H. and Liu, J. H.
Title: Modified Xiaochaihu Decoction () prevents the progression of chronic pancreatitis in rats possibly by inhibiting transforming growth factor-beta1/Sma- and mad-related proteins signaling pathway
Journal: Chin J Integr Med
Short Title: Modified Xiaochaihu Decoction () prevents the progression of chronic pancreatitis in rats possibly by inhibiting transforming growth factor-beta1/Sma- and mad-related proteins signaling pathway
Alternate Journal: Chinese journal of integrative medicine
ISSN: 1672-0415 (Print)
Accession Number: 24307314
Body Weight/drug effects
Drugs, Chinese Herbal/*therapeutic use
Real-Time Polymerase Chain Reaction
Signal Transduction/*drug effects
Transforming Growth Factor beta1/*metabolism
Abstract: OBJECTIVE: To investigate the effect of modified Xiaochaihu Decoction (, MXD) on transforming growth factor-beta1/Sma- and Mad-related proteins (TGF-beta1/Smads) signaling pathway in rats with chronic pancreatitis (CP) induced by dibutyltin dichloride. METHODS: Thirty healthy male Wistar rats were randomly divided into the normal control group, CP group and CP+MXD-treated group. CP was induced by injection of dibutyltin dichloride (DBTC, 7 mg/kg of body weight) into the right caudal vein, and the control rats were treated with vehicle. MXD was given daily by gavage at a dose of 10 g/kg of body weight, starting from the day after CP induction. After 28-day treatment, the n-benzoyl-tyrosyl para-aminobenzoic acid (NBT-PABA) test was carried out to evaluate exocrine pancreatic function. Then, rats were sacrificed, and pancreatic tissues were harvested for histological evaluation. In addition, the mRNA expression of TGF-beta1, TGF-beta1 type II receptor (TGFbetaRII), Smad3 and Smad7 was determined in pancreatic tissues by using real-time polymerase chain reaction. RESULTS: Treatment of CP with MXD improved the PABA recovery, decreased the histological lesion, and reduced the mRNA expression of TGF-beta1, TGFbetaRII and Smad3 (P<0.05). However, MXD had no effect on Smad7 mRNA level. CONCLUSIONS: MXD could protect the pancreas against chronic injury and improve pancreatic exocrine function in DBTC induced rat CP model. Its mechanism may involve inhibition of the TGF-beta1/Smads signaling pathway.