Pancreatitis

Notes: Sang, Gao

Du, Jian-Min

Chen, Yong-Yi

Chen, Yang-Bo

Chen, Jun-Xian

Chen, Yong-Can

eng

2013/11/05 06:00

Peptides. 2014 Jan;51:4-8. doi: 10.1016/j.peptides.2013.10.019. Epub 2013 Oct 30.

URL: http://www.ncbi.nlm.nih.gov/pubmed/24184419

Author Address: Department of Paediatrics, Tongde Hospital Zhejiang Chinese Medical University, 234 Gucui Road, Hangzhou 310012, China.

Department of Paediatrics, The First People’s Hospital of Hangzhou, Nanjing Medical University, 261 Huansha Road, Hangzhou 310006, China.

Department of Traditional Chinese Internal Medicine, Tongde Hospital Zhejiang Chinese Medical University, 234 Gucui Road, Hangzhou 310012, China. Electronic address: gstongde@63.com.

Intensive Care Unit, Tongde Hospital Zhejiang Chinese Medical University, 234 Gucui Road, Hangzhou 310012, China.

Department of Gastroenterology, Tongde Hospital Zhejiang Chinese Medical University, 234 Gucui Road, Hangzhou 310012, China.

 

 

Reference Type:  Journal Article

Record Number: 4769Author: Schick, V., Scheiber, J. A., Mooren, F. C., Turi, S., Ceyhan, G. O., Schnekenburger, J., Sendler, M., Schwaiger, T., Omercevic, A., Brandt, Cv, Fluhr, G., Domschke, W., Kruger, B., Mayerle, J. and Lerch, M. M.

Year: 2014

Title: Effect of magnesium supplementation and depletion on the onset and course of acute experimental pancreatitis

Journal: Gut

Volume: 63

Issue: 9

Pages: 1469-80

Date: Sep

Short Title: Effect of magnesium supplementation and depletion on the onset and course of acute experimental pancreatitis

Alternate Journal: Gut

ISSN: 1468-3288 (Electronic)

0017-5749 (Linking)

DOI: 10.1136/gutjnl-2012-304274

Accession Number: 24277728

Keywords: Acute Disease

Animals

Biological Markers/metabolism

Calcium/metabolism

Ceruletide

*Dietary Supplements

Disease Progression

Hydrolases/metabolism

Magnesium/metabolism/*therapeutic use

Magnesium Deficiency/*complications

Male

Mice

Pancreatitis/etiology/immunology/metabolism/*prevention & control

Peptide Hydrolases/metabolism

Rats

Rats, Wistar

Severity of Illness Index

Treatment Outcome

Abstract: BACKGROUND AND OBJECTIVE: High calcium concentrations are an established risk factor for pancreatitis. We have investigated whether increasing magnesium concentrations affect pathological calcium signals and premature protease activation in pancreatic acini, and whether dietary or intraperitoneal magnesium administration affects the onset and course of experimental pancreatitis. METHODS: Pancreatic acini were incubated with up to 10 mM magnesium; [Ca(2+)](i) (fura-2AM) and intracellular protease activation (fluorogenic substrates) were determined over 60 min. Wistar rats received chow either supplemented or depleted for magnesium (<300 ppm to 30 000 ppm) over two weeks before pancreatitis induction (intravenous caerulein 10 microg/kg/h/4 h); controls received 1 microg/kg/h caerulein or saline. C57BL6/J mice received four intraperitoneal doses of magnesium (NaCl, Mg(2+) 55 192 or 384 mg/kg bodyweight) over 72 h, then pancreatitis was induced by up to eight hourly supramaximal caerulein applications. Pancreatic enzyme activities, protease activation, morphological changes and the immune response were investigated. RESULTS: Increasing extracellular Mg(2+) concentration significantly reduced [Ca(2+)](i) peaks and frequency of [Ca(2+)](i) oscillations as well as intracellular trypsin and elastase activity. Magnesium administration reduced pancreatic enzyme activities, oedema, tissue necrosis and inflammation and somewhat increased Foxp3-positiv T-cells during experimental pancreatitis. Protease activation was found in animals fed magnesium-deficient chow-even with low caerulein concentrations that normally cause no damage. CONCLUSIONS: Magnesium supplementation significantly reduces premature protease activation and the severity of pancreatitis, and antagonises pathological [Ca(2+)](i) signals. Nutritional magnesium deficiency increases the susceptibility of the pancreas towards pathological stimuli. These data have prompted two clinical trials on the use of magnesium in patients at risk for pancreatitis.

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