November 12, 2014
ISSN: 0971-5916 (Print)
Accession Number: 24434269
Notes: Arora, Shalabh
Indian J Med Res. 2013 Oct;138(4):568.
Author Address: Department of Medical Oncology, Christian Medical College Hospital, Vellore, Tamil Nadu 632 004, India.
Reference Type: Journal Article
Record Number: 4603Author: Aruna, R., Geetha, A. and Suguna, P.
Title: Expression of caspase activation recruitment and pyrin domain levels of apoptosis-associated speck-like protein complex in the pancreas of rats subjected to experimental pancreatitis: Influence of rutin administration
Journal: Hum Exp Toxicol
Date: Nov 28
Short Title: Expression of caspase activation recruitment and pyrin domain levels of apoptosis-associated speck-like protein complex in the pancreas of rats subjected to experimental pancreatitis: Influence of rutin administration
Alternate Journal: Human & experimental toxicology
ISSN: 1477-0903 (Electronic)
Accession Number: 24287373
Abstract: The present study investigated the effect of rutin, a natural flavonoid, on the expression of caspase activation recruitment domain (CARD) and pyrin domain (PYD) of apoptosis-associated speck-like protein (ASC), a mediator of inflammation, in the pancreas of rats administered with ethanol (EtOH) and high-fat diet (HFD). Pancreatitis was induced in male albino Wistar rats by administering EtOH (8-12 g/kg/day) and HFD (22% fat) for 90 days. In addition, rats also received 100 mg rutin/kg body weight orally from 31st day till the experimental period. Serum levels of cytokines, interleukin 18 (IL-18) and IL-6; activity levels of caspase-1 and myeloperoxidase (MPO); messenger RNA (mRNA) expression of tumor necrosis factor alpha (TNF-alpha), caspase-1, CARD and PYD of ASC; and histological changes in pancreas were assessed. We observed a significant elevation in serum IL-18, IL-6, caspase-1 and MPO activities, mRNA expression of PYD, TNF-alpha and caspase-1 in the pancreas of rats administered with EtOH and HFD. Rutin administration along with EtOH and HFD significantly upregulated the mRNA expression of CARD and downregulated PYD, caspase-1, and TNF-alpha expressions. Rutin supplementation was also found to reduce IL-18 and IL-6 levels; and inflammatory changes in tissue architecture were evidenced by histological observations. The anti-inflammatory activity of rutin might be due to its effect on modulating the expression of ASC complex that mediates inflammation.