Dermatology 2013

Year: 2013

Title: Three-dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology

Journal: J Biophotonics

Volume: 6

Issue: 4

Pages: 352-62

Date: Apr

Short Title: Three-dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology

Alternate Journal: Journal of biophotonics

ISSN: 1864-0648 (Electronic)

1864-063X (Linking)

DOI: 10.1002/jbio.201200085

Accession Number: 22711418

Keywords: Carcinoma, Basal Cell/diagnosis/pathology

Cicatrix/diagnosis/pathology

Dermatitis, Seborrheic/diagnosis/pathology

Dermatology

Humans

Imaging, Three-Dimensional/*methods

Nevus/diagnosis/pathology

*Photons

Skin/*cytology/*pathology

Skin Diseases/*diagnosis/pathology

Skin Neoplasms/diagnosis/pathology

Tomography, Optical Coherence/*methods

Abstract: A preliminary clinical trial using state-of-the-art multiphoton tomography (MPT) and optical coherence tomography (OCT) for three-dimensional (3D) multimodal in vivo imaging of normal skin, nevi, scars and pathologic skin lesions has been conducted. MPT enabled visualization of sub-cellular details with axial and transverse resolutions of <2 mum and <0.5 mum, respectively, from a volume of 0.35 x 0.35 x 0.2 mm(3) at a frame rate of 0.14 Hz (512 x 512 pixels). State-of-the-art OCT, operating at a center wavelength of 1300 nm, was capable of acquiring 3D images depicting the layered architecture of skin with axial and transverse resolutions ~8 mum and ~20 mum, respectively, from a volume of 7 x 3.5 x 1.5 mm(3) at a frame rate of 46 Hz (1024 x 1024 pixels). This study demonstrates the clinical diagnostic potential of MPT/OCT for pre-screening relatively large areas of skin using 3D OCT to identify suspicious regions at microscopic level and subsequently using high resolution MPT to obtain zoomed in, sub-cellular level information of the respective regions.

Notes: Alex, Aneesh

Weingast, Jessika

Weinigel, Martin

Kellner-Hofer, Marcel

Nemecek, Romina

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